Patient has diabetes mellitus.
Anything more than 140 mg/dL is considered as diabetes mellitus.
1.If 0.5ml of serum is mixed with 1.0ml of water and a glucose test is performed resulting in a reading of 200mg/dl from the instrument, what is the final reported result?
2.If a 1:5 dilution is made with 0.5 ml of serum, how much water is used?
3.You are given a series of ten tubes, each of which contain 5ml of diluents. To the first tube is added 1ml of serum, and a serial dilution using 1ml is carried out in the remaining tubes. what is the serum concentration in tubes 4 and 8?
we are taking 0.2 mL of serum from tube 1.
We mix this into 5 mL of dilutent in tube 2. Then take 1 mL of solution from this and put it into tube 3, etc.
Each time we do this we are reducing the amount of serum by a factor of 5. So the amount of serum s, in each tube t is going to be of the form:
So in tube 4 we have
So the concentration in tube 4 is
and in tube 8 we have
So the concentration in tube 8 is