After using the following numbers to create a calibration curve

X

133406

418832

844875

y

0.0408

0.2221

0.4341

and adding a trendline the equation of the trendline is is

y = 0.000000548500693x - 0.023105816308584

How is it possible to use this to calculate the Y values for these X values?

39231.3

When I tried for the first number

y = 0.000000548500693(39231.3) - 0.02311

y = 0.0215183952372909 - 0.02311

y = -0.00159160 which can't be possible

when i'm guessing the answer should be around 0.0130

Thank you in advance