Hi,

I carried out 2 level 2 factorial design (factor A, high/low factor B, high/low), which gave four sets of conditions.

Due to the nature of the experiments I planned to run two batches with duplicates of each condition. However, due to equipment failures I ended up with different numbers of replicates across different batches, summarised in the table below. Do I need to make any adjustments for having different numbers of replicates for the whole data set and also different number of replicates within a batch?

Batch 1 Batch 2
A B A B
-1 -1 -1 -1
-1 1 -1 1
1 -1 1 -1
1 -1 1 -1
1 1 1 1
1 1 1 1

Thanks.